Very cheap, autonomous and precise spectrophotometer for DIY biology and chemistry.

This project is maintained by Hackuarium

E133 - Briliant blue

Exploratory experiments

The goal is to evaluate the concentration required to have an absorbance of around 0.5.

We took 427.0 mg in 100mL of water.

From this we placed 0.5mL in 100mL.

21 mg/L

Color Absorbance Emission
Red 1.04  
Green 0.04  
Blue 0.10  
UV   0

10.5 mg/L

Color Absorbance Emission
Red 0.61  
Green 0.05  
Blue 0.01  
UV   8


We need around 9 mg/L to have an absorbance in red of around 0.5.